Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate

Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate

CaptureSelect HRP Anti-AAVX Conjugate includes a 14-kDa camelid antibody fragment that specifically binds adeno-associated virus particles (AAV). The antibody fragment binds with high affinity to AAV particles and demonstrates good binding reactivity towards a variety of AAV serotypes, including AAV1 to AAV8, and AAVrh10. The antibody fragment is chemically conjugated to horseradish peroxidase (HRP) via a spacer of appropriate length to maintain full binding capacity of the antibody fragment.

CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (cat. no. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.