Thermo Scientific™ Syn-PER™ Synaptic Protein Extraction Reagent

Thermo Scientific™ Syn-PER™ Synaptic Protein Extraction Reagent

Thermo Scientific Syn-PER Synaptic Protein Extraction Reagent is a proprietary cell lysis reagent for efficient isolation of synaptosomes containing functional synaptic proteins from neuronal tissue and primary cultured neurons.

Syn-PER Reagent is used to prepare synaptosomes containing biologically active pre-and post-synaptic proteins (i.e., intact membranes and protein complexes of synapses). When used with fresh neuronal tissue or primary cultured neurons, synaptosomes prepared with the Syn-PER Reagent can be used to study synaptic transmission. The synaptosomal proteins contained in these extracts can also be used for downstream applications such as Western blotting, immunoprecipitation, enzymatic activity assays and protein-protein interaction studies. Syn-PER Synaptic Protein Extraction Reagent minimizes the degradation of phosphoproteins and is ideal for studies requiring the preservation of phosphoprotein integrity.

Features

• Efficient synapse extraction—obtain up to 10 µg of synaptic protein per milligram of neuronal tissue or 4 µg synaptic protein per 35mm dish of primary cultured neurons (106 cells)
• Gentle formulation—isolate viable synaptosomes; then lyse these synapses to extract native synaptic proteins and preserve phosphoprotein integrity
• Fast procedure—obtain synaptosomal suspension of intact synapses in less than one hour
• Simple protocol—requires no ultracentrifugation steps

Contents

• Syn-PER Synaptic Protein Extraction Reagent, 100mL, sufficient reagent to extract synaptic protein from ~10g of neuronal tissue and primary cultured neurons