Thermo Scientific™ CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line
On demandThermo Scientific™ CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line
The CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline- induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.
MoreThere are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/a059f90e-6987-43cf-8995-2960f1069cf5.jpg)
Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/1a2e0ce7-0cda-4484-9b6f-2ce7f74f731a.png)
Invitrogen™ LIVE/DEAD™ Fixable Near IR (780) Viability Kit, for 633 nm Excitation, 400 Assays
$ 649
On demand
![](https://laboshop.ae/public/storage/files/DOrRdOKQW1MISg3yIRxBxIBPz8F0F0CAiBfqf1ng.jpg)
Invitrogen™ Magnesium Green™, Pentapotassium Salt, cell impermeant
$ On demand