Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells, 20 Reactions
On demand
Order number :
K123120
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells, 20 Reactions
The CloneJET PCR Cloning Kit contains the novel, ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Re-circularized pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.
Features
- Fast—PCR cloning in only five minutes
- Highest efficiency—>99% of positive clones
- No cloning background—positive selection vector
- Versatile—ideal for blunt-end or sticky-end cloning
- Economical—no expensive blue/white screening
Contents
- 10 x DH10B Competent Cells for Subcloning (100 µL)
- pUC19 DNA (20 µL) (100 pg/µL)
- S.O.C. Medium (5 mL)
- pJET1.2/blunt Cloning Vector (24 µL) (50 ng/µL)
- 2X Reaction Buffer (300 µL)
- T4 DNA Ligase (24 µL) (5 U/µL)
- DNA Blunting Enzyme (24 µL)
- DNA Blunting Enzyme (24 µL)
- pJET1.2 Forward Sequencing Primer, 10 µM aqueous solution (50 µL)
- pJET1.2 Reverse Sequencing Primer, 10 µM aqueous solution (50 µL)
- Control PCR Product, 976 bp with 3’-dA overhangs (8 µL) (24 ng/µL)
- Water, nuclease-free, (1.25 mL)
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