Invitrogen™ DES™-Blasticidin Support Kit

Invitrogen™ DES™-Blasticidin Support Kit

The DES®-Blasticidin Support Kit is designed to be paired with any DES® Expression vector to allow rapid selection of stable S2 cells with Blasticidin. The DES®-Blasticidin Support Kit contains the pCoBlast selection vector for rapid generation of stable S2 cell lines with Blasticidin. When this kit is paired with pMT/V5-His, pMT/V5-His-TOPO®, pMT-DEST48, pMT/BiP/V5-His, or pAc5.1/V5-His, the vector and the DES®-Blasticidin Support Kit form a complete system for stable expression in Drosophila S2 cells.

Contents

• pCoBlast vector: 40 μL at 0.5 μg/μL in TE, pH 8 
• Blasticidin: 50 mg, powder  
• Schneider (S2) Cells: 1 vial containing 1 mL of cells at 1 × 107 cells/mL in Freezing Medium 
• Schneider’s Drosophila Medium: 2 × 500 mL