Thermo Scientific™ DSBU (Disuccinimidyl Dibutyric Urea), 10 x 1 mg
On demandThermo Scientific™ DSBU (Disuccinimidyl Dibutyric Urea), 10 x 1 mg
DSBU (disuccinimidyl dibutyric urea), or BuUrBU, is a mass-spectrometry (MS) -cleavable and membrane-permeable cross-linker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 11-atom spacer arm. The cross-linker facilitates analysis of protein structure and complex interactions using mass spectrometry. DSBU has similar reactivity to DSS, but contains a urea linker that can be cleaved in the gas phase during tandem MS (MS/MS) using collision-induced dissociation (CID). The ability to cleave cross-linked peptides during MS/MS enables MS3 acquisition methods, which facilitate peptide sequencing using traditional database search engines. The MS cleavage of DSBU also generates diagnostic ion doublets during MS2, which enables identification of cross-linked peptides from dead-end modifications and searching using novel database search engines such as MeroX or XlinkX.
Features
- Reactive group: NHS ester (both ends)
- Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
- Membrane permeable, allowing for intracellular crosslinking
- High-purity, crystalline reagent can be used to create high-purity conjugates
- MS-cleavable
- Water-insoluble (dissolve first in DMF or DMSO)
- Purity: > 90% by quantitative NMR (the highest standard in crosslinking purity)
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