Thermo Scientific™ DyLight™ 488 Maleimide

Thermo Scientific™ DyLight™ 488 Maleimide

Thermo Scientific DyLight 488 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 488 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 488 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2™ dye, Alexa Fluor™ 488, FITC, and LI-COR™ dyes in many applications. The high water solubility of DyLight Fluors allows a high dye-to-protein ratio to be achieved without causing precipitation of the conjugates.

Features

• High performance—DyLight 488 shows brighter fluorescence than Alexa Fluor 488, FITC and Cy2 dye
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency