Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit, 1000 Reactions

Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit, 1000 Reactions

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

For use with: Luminometer (Microplate)

Features

• Sensitive—highly sensitive detection of Cypridina luciferase activity
• Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
• Stable—reagents provide increased signal stability compared to flash assays
• Simple—does not require a luminometer with injectors
• Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
• Automation-friendly—amenable to high throughput assays
• Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
• Safe—allows one to perform non-radioactive assays

Contents

• Cypridina Glow Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 0.5 mL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature