Thermo Scientific™ EZ-Link™ NHS-SS-PEG4-Biotin

Thermo Scientific™ EZ-Link™ NHS-SS-PEG4-Biotin

Thermo Scientific EZ-Link NHS-SS-PEG4-Biotin includes disulfide and polyethylene glycol (PEG) groups in its spacer arm to provide biotinylation that is cleavable and which enhances solubility.

NHS-SS-PEG4-Biotin enables simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules. The N-hydroxysuccinimide ester (NHS ester) group reacts specifically and efficiently with protein and peptide lysine (K) and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The integral disulfide bond enables the biotin group to be cleaved from the labeled molecule using DTT or other reducing agents, providing for efficient recovery of avidin-bound protein complexes in affinity-based assays.

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
• Pegylated—spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Long reach—spacer arm (total length added to target) is 37.9 angstroms; this reduces steric hindrance when binding to avidin molecules