Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO

Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO

EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach. The DBCO group reacts preferentially with an azide through a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form a stable triazole linkage with an azide-labeled coupling partner. EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and reach.

Features

• Bioorthogonal—DBCO and azides react selectively to form triazole linkages
• Enhanced solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms irreversible and stable triazole linkage