Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO
On demand
Order number :
C20042
Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO
EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach. The DBCO group reacts preferentially with an azide through a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form a stable triazole linkage with an azide-labeled coupling partner. EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and reach.
Features
- Bioorthogonal—DBCO and azides react selectively to form triazole linkages
- Enhanced solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
- Irreversible—forms irreversible and stable triazole linkage
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