Thermo Scientific™ EZ-Link™ Desthiobiotinylation and Pull-Down Kit

Thermo Scientific™ EZ-Link™ Desthiobiotinylation and Pull-Down Kit

The Thermo Scientific EZ-Link Desthiobiotinylation and Pull-Down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

Features

• Sulfo-NHS-LC-Desthiobiotin – label proteins with a biotin-like that specifically binds streptavidin but also easily elutes with mild conditions
• Pull-down assay – simple, optimized protocol and reagents to label proteins and recover (purify) intact, functional protein interaction complexes
• Complete kit – includes labeling reagent and desalting columns to prepare bait-proteins, and streptavidin agarose and elution buffer to perform pull-down

Contents

Sufficient For: 5 labeling reactions (0.2-5 mg protein each) and up to 40 pull-down experiments (50 µL each)

• EZ-Link Sulfo-NHS-LC-Desthiobiotin: 2 x 1 mg
• Zeba Spin Desalting Column (7K MWCO): 5 mL, 5 columns
• High Capacity Streptavidin Agarose Resin: 2 mL
• PBS Pack (makes 500mL): 1 pack
• Biotin Elution Buffer: 8 mL