Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

Features

• GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins from many different sources
• Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin

Contents

Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin

• Glutathione Agarose: 750 µL
• Pull-Down Lysis Buffer: 250 mL
• Glutathione: 1 g
• TBS Pack (makes 500 mL): 1 pack
• Pierce Spin Columns and Accessories: 27 columns
• Microcentrifuge Collection Tubes: 100 tubes