Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

Features

• Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
• Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
• Adaptable to single- or multiple-sample demands
• Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads

Contents

Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin

• Streptavidin Agarose Resin: 1.5 mL
• TBS Pack (makes 500mL): 1 pack
• Biotin Blocking Solution: 15 mL
• Wash Buffer (pH 5.0): 100 mL
• Elution Buffer (pH 2.8): 50 mL
• Pierce Spin Columns and Accessories: 27 columns
• Microcentrifuge Collection Tubes: 200 tubes