Thermo Scientific™ EZ-Link™ Pentylamine-Biotin
On demandThermo Scientific™ EZ-Link™ Pentylamine-Biotin
Thermo Scientific EZ-Link Pentylamine-Biotin is biotin that has been conjugated to pentylenediamine to add a short, amine-terminated spacer to its valeric acid group, providing a simple amine-modified compound for different applications.
Pentylamine Biotin is a compound formed by modification of the valeric acid side chain of biotin with pentane-1,5-diamine. The compound contains terminal primary amine (-NH2), which provides a functional handle for derivatization or conjugation to proteins, surfaces and other molecules. Carbodiimide (EDC) and NHS-ester crosslinker chemistries are most often utilized for covalent modifications involving amino-biotin molecules. The compound is also useful as an amine-alternative to native (carboxylate) biotin for assays and methods that require a biotin standard or blocking step.
Features
- Biotinylation—label molecules and surfaces for assay or affinity purification methods involving avidin or streptavidin probes and resins
- Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
- Medium length—spacer arm (total length added to target) is 18.9 angstroms, representing a 6-atom extension of the native biotin valeric acid (and its conversion to a primary amine)
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