Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit
On demand
Order number :
21445
Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit
The Thermo Scientific EZ-Link Sulfo NHS-SS Biotinylation Kit contains sufficient reagents for 10 biotinylation reactions (e.g., 1–10 mg antibody per reaction). The EZ-Link Sulfo-NHS-SS-Biotin included in the kit is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.
Features
- Protein labeling – biotinylate antibodies to facilitate immobilization, purification or detection
- Cell surface labeling – biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
- Soluble – charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Medium length—spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
Contents
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
- EZ-Link Sulfo-NHS-SS-Biotin: 25 mg
- PBS Pack (makes 500 mL): 1 pack
- Zeba Spin Desalting Columns (7K MWCO): 5 mL, 10 columns
- HABA Solution: 1 mL
- Avidin: 10 mg
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