Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

The Thermo Scientific EZ-Link Sulfo-NHS-Biotinylation Kit contains reagents sufficient for 10 biotinylation reactions (e.g., 1–10 mg antibody per reaction). The EZ-Link Sulfo-NHS-Biotin reagent included in the kit is a short-chain, water-soluble biotinylation reagent used for labeling antibodies, proteins and other molecules that have primary amines.

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.

Contents

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody

• EZ-Link Sulfo-NHS-Biotin: 25 mg
• PBS Pack (makes 500mL): 1 pack
• Zeba Spin Desalting Columns (7K MWCO: 5 mL, 10 columns
• HABA Solution: 1 mL
• Avidin: 10 mg