Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit
On demand
Order number :
21435
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit
The Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotinylation Kit contains reagents sufficient for 10 biotinylation reactions (e.g., 1–10 mg antibody per reaction). The EZ-Link Sulfo-NHS-LC-Biotin reagent included in the kit is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins, and other molecules that have primary amines.
Features
- Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Contents
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
- EZ-Link Sulfo-NHS-LC-Biotin: 25 mg
- PBS Pack (makes 500 mL): 1 pack
- Zeba Spin Desalting Columns (7K MWCO): 5 mL, 10 columns
- HABA Solution: 1 mL
- Avidin: 10 mg
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