Thermo Scientific™ FDAA (Marfey's Reagent) (1-fluoro-2-4-dinitrophenyl-5-L-alanine amide)

Thermo Scientific™ FDAA (Marfey's Reagent) (1-fluoro-2-4-dinitrophenyl-5-L-alanine amide)

Thermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

FDAA is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide, also called Marfey's reagent. The compound reacts with primary amines and is used as a derivatization reagent for UV detection in liquid chromatography methods. The reagent provides for detection at 340nm with nanomole sensitivity. FDAA derivatives of D-amino acids exhibit strong intramolecular bonding, which reduces their polarity relative to the corresponding L-amino acid derivatives. Consequently, the D-derivatives are selectively retained on reverse phase columns and elute much later than corresponding L-derivatives.

Features

• For precolumn derivatization of primary amines for detection by UV absorbance (λmax = 340nm)
• Provides nanomole sensitivity in typical HPLC applications with amino acids
• Reaction products enable chiral separation of D- and L-amino acids
• Following reverse-phase HPLC, derivatives have an extinction coefficient of 3 x 10^4M-1 cm-1