Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)
On demandThermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)
Thermo Scientific Pierce Fluoraldehyde OPA Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent derivatization reagent for peptide or amino acid detection and quantitation in HPLC.
Fluoraldehyde Reagent Solution contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.
Features
- For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
- Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
- Provides an accurate measure of both composition and absolute protein-peptide content
- Ideal for work with recombinant proteins and synthetic peptides
- Can be used for fluorescent protein or peptide assay
- Pre-column derivatization mixtures can be injected into LC without any processing
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