Thermo Scientific™ GlycoLink™ Immobilization Kit

Thermo Scientific™ GlycoLink™ Immobilization Kit

The Thermo Scientific GlycoLink Immobilization Kit contains affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.

Features

• High-capacity—immobilize 1 to 10 mg of oxidized antibody or other glycoprotein per milliliter of resin
• Fast and efficient—immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
• Specific immobilization—the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
• Stable attachment—resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
• Preserves antibody function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target

Contents

• GlycoLink Columns, 1 mL: 10 columns
• GlycoLink Coupling Buffer: 2 x 100 mL
• Aniline: 0.2 mL
• Wash Buffer: 120 mL
• Sodium meta-periodate: 0.5 g
• Zeba Spin Desalting Columns, 5 mL: 10 columns
• PBS Pack (makes 500mL): 1 pack
• Column Accessories: 10 each