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Thermo Scientific™ HisPur™ Cobalt Purification Kit, 1 mL

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Order number : 90091
$ On demand

Thermo Scientific™ HisPur™ Cobalt Purification Kit, 1 mL


The Thermo Scientific HisPur Cobalt Purification Kit contains 0.2-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.


Features

  • High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
  • Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
  • Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
  • Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
  • Cost effective—resin can be reused several times or discarded after single use
  • Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates
  • Format: 15 mL conical, Kit, Spin Column
  • Stationary Phase: Metal Chelate, Divalent Cobalt


Contents

Sufficient For: Purifying ∼10 mg of His-tagged protein per column

  • HisPur Cobalt Spin Columns, 1 mL, 5 columns
  • Equilibration/Wash Buffer, 100 mL
  • Elution Buffer, 50 mL


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