Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL

The Thermo Scientific HisPur Ni-NTA Spin Purification Kit contains 0.2-ml HisPur Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins. HisPur Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features

• High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
• Cost-effective—reuse the same batch of resin at least five times
• Easy to use—pre-formulated buffers available for kit formats
• Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates
• Format: 50 mL conical, Kit, Spin Column
• Stationary Phase: Divalent Nickel, Metal Chelate

Contents

Sufficient For: Purifying up to 180 mg of His-tagged protein per column

• HisPur Ni-NTA Spin Columns, 3 mL, 5 columns
• Phosphate-buffered Saline (10X), 30 mL
• Imidazole (2M), 25 mL
• Column Plugs, 5 plugs