Thermo Scientific™ L-Photo-Leucine

Thermo Scientific™ L-Photo-Leucine

Thermo Scientific Pierce L-Photo-Leucine is an analog of L-leucine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

Features

• In vivo labeling—incorporate photo-reactive group into proteins using normal cellular machinery of live cells
• In vivo crosslinking—find interacting proteins in the native cellular environment
• Increased specificity—crosslink interacting proteins correctly positioned at their interfaces within protein interaction domains
• Efficient recovery—90% protein recovery in cell lysates after crosslinking
• Compatible—crosslink proteins expressed in a wide variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3 and C6
• Easy to use—reagents are photo-stable under normal laboratory lighting, eliminating the need to work in the dark