Thermo Scientific™ MCF7 (+hIGF-1) Control Lysate (1mg/mL)

Thermo Scientific™ MCF7 (+hIGF-1) Control Lysate (1mg/mL)

Thermo Scientific MCF7 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway.

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This lysate has been validated for expression of ten key proteins and phosphorylation in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2).

For use with equipment: Mass Spectrometer

Features

• Validated for the detection and phosphorylation of AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 proteins
• Optimized—stimulation and lysis conditions optimized for detection of AKT signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C