Thermo Scientific™ Micro BCA™ Protein Assay Kit

Thermo Scientific™ Micro BCA™ Protein Assay Kit

The Pierce Micro BCA Protein Assay Kit is a three-component version of our BCA reagents, optimized to measure total protein concentration of dilute protein solutions (0.5 to 20 µg/mL).

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

For use with equipment: Spectrophotometer, Microplate Reader

Features

• Sensitivity—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
• Working range—linear working range for BSA of 0.5 to 20 µg/mL
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Stability—kit is stable at room temperature; prepared working reagent stable for up to 24 hours

Contents

• Micro BCA Reagent A (MA): 240 mL
• Micro BCA Reagent B (MB): 240 mL
• Micro BCA Reagent C (MC): 12 mL
• Albumin Standard Ampules, 2 mg/mL: 10 x 1 mL