Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit, 1000 Reactions

Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit, 1000 Reactions

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.

For use with: Luminometer (Microplate)

Features

• Sensitive—highly sensitive detection of Gaussia luciferase activity
• Stable—increased signal stability
• Simple—does not require a luminometer with injectors
• Compatible—assay reagents compatible with other Gaussia luciferases
• Automation-friendly—amenable to high throughput assays
• Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
• Safe—allows one to perform non-radioactive assays

Contents

• Gaussia Glow Assay Buffer, 50 mL (store at 4°C)
• 100X Coelenterazine, 0.5 mL (store at -80°C)
• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)