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Thermo Scientific™ pCMV-Green Renilla Luc Vector for Luciferase Assays

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Order number : 16153
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Thermo Scientific™ pCMV-Green Renilla Luc Vector for Luciferase Assays


The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the CMV (Cytomegalovirus) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.


Features

  • Strong (CMV) constitutive promoter for co-transfection and normalization
  • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin


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