Thermo Scientific Phusion™ Plus DNA Polymerase, 100

Thermo Scientific Phusion™ Plus DNA Polymerase, 100

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.

Features

• High fidelity—provides >100X higher sequence accuracy than Taq polymerase
• Convenient setup—enables a universal annealing temperature of 60°C in PCR
• Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation
• Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
• High inhibitor tolerance—works with DNA of suboptimal purity

Contents

• Phusion Plus DNA Polymerase, 50 µL
• 5X Phusion Plus Buffer, 1.25 mL
• 5X Phusion GC Enhancer, 1.25 mL