Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin, 1 mL

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin, 1 mL

Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport. UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity. The porosity, rigidity, and durability of the resin make it suitable for medium-pressure, fast-flow techniques involving large sample volumes.

Features

• Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
• High purity—optimized bind-wash-elute protocol enables high purity
• High yield—special antibody conjugation method enables high yield
• Rapid—entire purification protocol typically takes less than 40 mins
• Economical—purification protocol allows multiple reuses
• Versatile—can be used in spin-, gravity-, or FPLC-based purification and enrichment methods
• Scalable—available in 1, 5, and 50 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purifications

Contents

• 1 mL settled resin. 2 mL supplied as a 50% slurry in PBS containing 0.02% sodium azide