Thermo Scientific™ Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit

Thermo Scientific™ Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit

The Thermo Scientific Pierce Magnetic c-Myc-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

Unlike traditional IP procedures based on capture with Protein A/G beads, this kit uses magnetic beads containing pre-immobilized anti-c-Myc antibody. These Pierce Anti-c-Myc Magnetic Beads ensure specific binding of c-Myc tagged protein complexes from biological samples. Because the antibody is covalently attached to the beads, IP-targets are easily eluted and recovered without antibody contamination. The complete IP kit includes the magnetic beads, lysis/wash buffer, low-pH elution buffer, neutralization buffer, c-Myc-tag positive control lysate, and non-reducing sample buffer for SDS-PAGE. Protocols are provided for both manual and automated magnetic separation workflows. Sufficient components are provided to perform 40 IP or co-IP assays.

For use with equipment: KingFisher™ Flex Magnetic Particle Processor, Magnetic Stand

Features

• Specific magnetic beads—covalently immobilized high-quality anti-c-Myc monoclonal antibody enables high yields of immunoprecipitation products
• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding
• Trouble-free elution—low-pH elution buffer ensures recovery of c-Myc-tagged protein interaction complexes without antibody leaching contamination
• Convenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour
• Versatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments)

Contents

• c-Myc-tagged Positive Control (Part No. 23633): 500 µL
• Pierce Anti-c-Myc Magnetic Beads: 1 mL
• Mag c-Myc IP/Co-IP Buffer-1: 50 mL
• Mag c-Myc IP/Co-IP Buffer-2 (20X): 20 mL
• Non-Reducing Sample Buffer (5X): 5 mL
• Elution Buffer (pH 2.0): 5 mL