Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit, 100 Reactions

Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit, 100 Reactions

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animated videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

For use with: Luminometer (Microplate)

Features

• Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
• Sensitive—measure bright blue Cypridina and red firefly luciferase activities in the same sample
• Fast—no quenching step required, unlike in traditional sequential dual assays
• Multiplex—capable of quantitating two cellular activities in the same sample(s)

Contents

• Cypridina-Firefly Dual Assay Buffer, 5 mL (store at 4°C)
• 100X Vargulin, 50 µL (store at -20°C)
• D-Luciferin, 3 mg (store at 4°C)
• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)