Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit

Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit

Thermo Scientific Pierce Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Magnetic IP with NHS chemistry is convenient and allows for the immobilization of IP antibody from any species and subtype without the variations in coupling efficiency seen with Protein A and Protein G. The IP antibody is covalently attached so target proteins or co-IP protein complexes can be eluted and analyzed without antibody contamination. The kit contains an optimized protocol and all buffers and reagents necessary for antibody coupling and high yield IP or co-IP using either manual magnetic stands or automated platforms such as the Thermo Scientific KingFisher Instruments.

For use with equipment: KingFisher™ Flex, Magnetic Stand

Features

• Antibody-free IP—antibody is irreversibly attached to the magnetic beads, resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen
• Fast—complete antibody conjugation and immunoprecipitation in less than 4 hours
• Convenient—the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation
• Antibody compatible—use with any antibody species, class or isotype
• Low nonspecific binding—the bead surface is pre-blocked and all nonreacted NHS-ester groups are fully quenched
• Protocol-compatible—protein coupling to the beads and immunoprecipitation can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)
• Scalable—use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody magnetic beads for multiple experiments

Contents

• Pierce NHS-Activated Magnetic Beads: 1 mL
• IP Lysis/Wash Buffer: 2 x 50 mL
• Elution buffer, pH 2.0: 5 mL
• Lane Marker Sample Buffer, Non-reducing, (5X): 5 mL
• Neutralization Buffer, pH 8.5: 0.5 mL
• 0.67M Borate Buffer: 1 mL
• BupH Borate Buffer Pack: 1 pack
• Quenching Buffer: 25 mL