Thermo Scientific™ Pierce™ Kinase Enrichment Kit with ATP Probe
On demand
Order number :
88310
Thermo Scientific™ Pierce™ Kinase Enrichment Kit with ATP Probe
This Thermo Scientific Pierce Kinase Enrichment Kit utilizes an ActivX™ ATP Probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.
ActivX ATP Probes feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.
Features
- Specific – label only the conserved active-site lysines of nucleotide-binding proteins
- Flexible – use for in vitro labeling of ATPase enzymes derived from cells or tissues
- Compatible – use with Western blot or mass spectrometry (MS) workflows
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