Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit

Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit

Thermo Scientific Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

This microplate-based biotin assay is easy to perform by adding the supplied fluorescent reporter to the biotinylated samples and diluted biocytin standards. The avidin fluoresces when the weakly interacting HABA (4'-hydroxyazobenzene-2-carboxylic acid) is displaced by the biotin. The amount of biotin is determined by comparing the sample's fluorescence to the biocytin standard curve. This assay requires must less sample volume than the microplate colorimetric HABA assay and is much more sensitive.

Features

• Fast—requires only 5 minute incubation
• Economical—requires only 10 µL of sample (approx. 750ng of biotinylated IgG)
• Sensitive—linear working range of 10 to 60 picomoles of biotin

Contents

Sufficient for 216 samples in microplate format

• Phosphate Buffered Saline (20X): 3 mL
• DyLight Reporter: 1.3 mL
• 1 mM Biocytin Control: 0.1 mL