Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

This Pierce Microplate BCA Protein Assay Kit is the reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain disulfide reducing agents and comes with twenty 96-well microplates.

The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, BME, or TCEP. This reducing agent-compatible BCA kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

For use with equipment: Spectrophotometer, Microplate Reader

Features

• Compatibility—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
• BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
• Sample volume—requires only 25 µL (standard kit) or less than 10 µL (microplate kit) of sample
• Colorimetric—measure with a standard spectrophotometer or plate reader (562 nm)
• Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Assay range—linear working range for BSA of 125 to 2000 µg/mL

Contents

• BCA Reagent A: 250 mL
• BCA Reagent B: 25 mL
• Compatibility Reagent: 48 x 10 mg
• Reconstitution Buffer: 15 mL
• Albumin Standard Ampules, 2mg/mL: 10 x 1 mL
• 96-Well Microplates: 20 plates