Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

The Thermo Scientific Pierce Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups. SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.

Features

• Adds a protected sulfhydryl that can be deprotected by hydroxylamine
• Allows long-term storage of the sulfhydryl-modified molecule
• Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
• Reacts with primary amines (e.g., lysine residues proteins) to form stable amide bonds
• Preserves protein activity with its mild, non-denaturing reaction conditions