Thermo Scientific™ pJTI™ R4 Dest CMV N-EmGFP pA Vector

Thermo Scientific™ pJTI™ R4 Dest CMV N-EmGFP pA Vector

The pJTI™ R4 Dest CMV N-EmGFP pA vector allows a gene of interest to be expressed as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line. When used in combination with a Jump-In™ parental cell line kit such as the Jump-In™ GripTite™ HEK293 cell line (A14150), an isogeneic stable cell line can be created with less effort and in less time than traditional cell engineering methods. In addition, Gateway® technology increases the efficiency of the cloning steps to introduce your GOI into the Gateway®-ready pJTI™ R4 Dest CMV N-EmGFP pA vector. The high retargeting efficiency, made possible by the R4 sites in the Jump-In™ parental cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest with an N-terminal EmGFP.

Features

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods.
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms or orthologs.
• Generate multiple cell lines in parallel due to the simplified work flow.
• Easily access the Jump-In™ technology with the ability to generate an unlimited number of cell lines without complicated licenses or restrictions to interpret.