Thermo Scientific™ Pro-Detect™ Rapid DYKDDDDK Competitive Assay Kit
On demand
Order number :
A38508
Thermo Scientific™ Pro-Detect™ Rapid DYKDDDDK Competitive Assay Kit
The Thermo Scientific Pro-Detect Rapid DYKDDDDK Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of DYKDDDDK (commonly known as 'FLAG')-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to DYKDDDDK tag to provide a qualitative detection within ten minutes. This assay can be used to detect DYKDDDDK-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features
- Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
- Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
- Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
- Specific—assay does not cross-react with common lysis and protein preparation reagents
- Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
- Long shelf life—stable for at least one year at 4°C
Contents
- 10 Rapid Assay Strips
- Sample Diluent, 15 mL
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