Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Thermo Scientific Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours.

Subcellular Protein Fractionation Kit for Tissue includes a unique 250 µM tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization. The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination.

Features

• Optimized—formulations and protocols specific for fractionation of tissue
• Complete—obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
• Time efficient—extract functional protein fractions without ultracentrifugation in approx. 2 hours
• Convenient—unique tissue strainer allows rapid removal of tissue debris
• Compatible—use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays.
• Versatile—extract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen

Contents

• Cytoplasmic Extraction Buffer (CEB) for Tissues: 50mL
• Membrane Extraction Buffer (MEB) for Tissues: 35mL
• Nuclear Extraction Buffer (NEB) for Tissues: 20mL
• Pellet Extraction Buffer (PEB) for Tissues: 6.5mL
• Micrococcal Nuclease, ≥ 100 units/µL: 260µL
• Calcium Chloride (CaCl2), 100mM: 450µL
• Halt Protease Inhibitor Cocktail, 100X: 1mL
• Pierce Tissue Strainer, 250µm: 25 devices