Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Kit - Western Blot Application

Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Kit - Western Blot Application

The Thermo Scientific Pierce Sulfo-SBED Biotin Label Transfer Reagent is a multifunctional reagent for labeling a purified protein and then covalently transferring the attached biotin tag onto specific interactors of that protein.

Sulfo-SBED is the abbreviation for Sulfo-N-hydroxysuccinimidyl-2-(6-[biotinamido]-2-(p-azido benzamido)-hexanoamido) ethyl-1,3'-dithioproprionate. It is a heterobifunctional chemical crosslinker capable of covalently attaching to primary amines at one end and to nearly any protein functional group at the other end. Unlike typical crosslinkers, Sulfo-SBED also includes a biotin group and a cleavable disulfide spacer arm. Together these features allow one to sequentially crosslink interacting proteins and transfer the biotin affinity tag from one protein (i.e., a purified 'bait' protein) to another (possibly unknown 'prey' protein). Label Transfer is a powerful in vitro method for protein interaction discovery. A growing number of publications feature the use of Sulfo-SBED Biotin Label Transfer Reagent to identify previously unknown protein interaction binding partners and to more fully characterize the specific protein binding domains of other protein interactions.

Contents

Sufficient For: 8 label transfer reactions for subsequent western blot analysis

• Sulfo-SBED, 8 x 1 mg
• PBS pack (makes 500 mL), 1 pack
• Label Transfer Buffer (20X), 200 mL
• Streptavidin-HRP Conjugate, 0.1 mg
• Dithiothreitol (DTT), 8 x 7.7 mg
• Slide-A-Lyzer MINI Dialysis Devices Plus Float, 10K MWCO, 10-device kit