Thermo Scientific™ SureQuant™ AKT Pathway (Phospho) Absolute Quantitation Module
On demandThermo Scientific™ SureQuant™ AKT Pathway (Phospho) Absolute Quantitation Module
The Thermo Scientific SureQuant AKT Pathway (Phospho) Absolute Quantitation Module provides a complete quantitation solution for the analysis of key phosphorylation events of AKT signaling pathway proteins using mass spectrometry (MS).
The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key phosphorylated proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.
For use with: Thermo Scientific™ Q Exactive Mass Spectrometer, Mass Spectrometer
Features
- Complete—contains validated AQUA HeavyPeptide and LightPeptide mixtures, diluent, matrix, system suitability standard including optimized acquisition and analysis methods
- Validated—peptides rigorously tested for successful quantitation of each target peptide
- Multiplex—able to quantitate phosphorylated AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction
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