Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.

Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.

Features

• Concise—extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions from mammalian cultured cells in less than 3 hours
• Convenient—perform a simple procedure without using ultracentrifugation over gradients
• Complete—obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
• Cultured Cells—developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues
• Compatible—use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays.

Contents

• Cytoplasmic Extraction Buffer (CEB): 10mL
• Membrane Extraction Buffer (MEB): 10mL
• Nuclear Extraction Buffer (NEB): 10mL
• Pellet Extraction Buffer (PEB): 5mL
• Micrococcal Nuclease, ≥ 100 units/µL: 150µL
• Calcium Chloride (CaCl2), 100mM: 250µL
• Halt™ Protease Inhibitor Cocktail, 100X: 350µL