Thermo Scientific™ T4 DNA Ligase, HC (30 U/µL)
On demand
Order number :
EL0013
Thermo Scientific™ T4 DNA Ligase, HC (30 U/µL)
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
T4 DNA Ligase requires ATP as a cofactor.
Features
- Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
- Fast—sticky-end ligation is completed in 10 minutes at room temperature
- Supplied with PEG solution for efficient blunt-end ligation
Contents
- T4 DNA Ligase
- 10X T4 DNA Ligase Buffer
- 50% PEG Solution