Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial, 200 Reactions

Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial, 200 Reactions

The Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. This version of the kit comes with a separate vial of ROX reference dye.

For use with: BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon

Features

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

Contents

• Verso Enzyme Mix: 1 x 50 µL
• RT enhancer: 1 x 250 µL
• 2X 1-Step qPCR Mix: 2 x 1.25 mL
• ROX, 1mM: 1 x 25µL
• Sufficient for 200 x 25 µL reactions.