Invitrogen™ FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination

Invitrogen™ FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination

The FISH Tag™ RNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ RNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for the simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts. We also offer FISH Tag™ Kits in single colors.
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

Features

• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success

Contents

• Alexa Fluor® reactive dye (Component A): 5 vials in each of 2 pouches
• Dimethylsulfoxide (DMSO) (Component J): 200 μL
• Dithiothreitol (DTT) (Component H): 100 μL
• Glycogen, ultrapure, nuclease free (Component I): 20 μL
• Sodium bicarbonate (Component K): 84 mg
• Water, nuclease free (Component L): 4.0 mL
• RNA nucleotide mix (Component M): 20 μL
• T7 RNA polymerase (Component N): 10 μL
• T3 RNA polymerase (Component O): 10 μL
• SP6 RNA polymerase (Component P): 10 μL
• DNase I (Component Q): 15 μL
• T3/T7 transcription buffer (Component R): 80 μL
• SP6 transcription buffer (Component S): 40 μL
• RNaseOUT™ ribonuclease inhibitor (recombinant) (Component T): 10 μL
• SlowFade® Gold antifade reagent (Component G): 2 mL
• Binding buffer (Component B): 6.0 mL
• Wash buffer (Component C): 3.2 mL
• Elution Buffer (Component D): 6.0 mL
• Spin columns and collection tubes (Component E): 20 columns with tubes
• Collection tubes (Component F): 20 tubes