Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient
  
  

Contents

• 5X TdT Reaction Buffer: 1 mL
• Terminal Deoxynucleotidyl Tranferase: 5 µL
• Biotin-11-UTP: 1 µL
• Unlabeled Control Oligo: 14 µL
• Biotin-Control Oligo: 4 µL