Invitrogen™ FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye

Invitrogen™ FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

Features

• Dye (Ex/Em): Alexa Fluor® 555 (555/565 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success
  
  

Contents

• Alexa Fluor® reactive dye (Component A):5 vials in each of 2 pouches
• Dimethylsulfoxide (DMSO) (Component J): 200 μL
• Dithiothreitol (DTT) (Component H): 100 μL
• Glycogen, ultrapure, nulcease free (Component I): 20 μL
• Sodium bicarbonate (Component K): 84 mg
• Water, nuclease free (Component L): 4.0 mL
• DNA nucleotide mix (Component M): 80 μL
• DNA polymerase I (Component P): 25 μL 1
• DNase I (Component O): 15 μL
• Nick translation buff  er (Component N): 250 μL
• SlowFade® Gold antifade reagent (Component G): 2 mL
• Binding buff  er (Component B): 6.0 mL
• Wash buff  er (Component C): 3.2 mL
• Elution buff  er (Component D): 6.0 mL
• Spin columns and collection tubes (Component E):20 columns with tubes NA
• Collection tubes (Component F): 20 tubes