Invitrogen™ SuperScript™ Plus Direct cDNA Labeling System with Alexa Fluor™-aha-dUTPs

Invitrogen™ SuperScript™ Plus Direct cDNA Labeling System with Alexa Fluor™-aha-dUTPs

The SuperScript® Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling. In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor® 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents. 

Features

• Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor® nucleotides, enabling higher numbers of array positives
• Improved correlation coefficients for more accurate data 
• Low elution purification columns included for ease of use

Contents

• All SuperScript™ Direct cDNA Labeling Systems/Modules include SuperScript™ III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript™ Plus Direct cDNA Labeling System/Module additionally include Alexa Fluor™ 555- and 647-aha-dUTP. The 'System' also includes purification components.