Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapy, cell-based vaccine, and similar biotherapeutics. Reliable and rapid, the resDNASEQ kit enables sensitive and specific quantitation of different fragment lengths of the E1A gene within the residual host-cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to purified final product—whether the sample contains high molecular weight or sheared DNA.

Features

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, three TaqMan primer/probe mixes, and a single E1A plasmid DNA standard

Contents

resDNASEQ E1A DNA Control

• E1A DNA Control, 6.30E+08 copies/μL, 0.12 mL, store at –25 to –15°C
• DNA Dilution Buffer (DDB), 7 mL, store at –25 to –15°C before first use and 2−8°C after first use

resDNASEQ E1A DNA Real-Time PCR Reagents

• 2X Environmental Master Mix, 5 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• E1A DNA Short Fragment Assay Mix, 1 x 400 μL, store at –15 to –25°C, protect from light
• E1A DNA Medium Fragment Assay Mix, 1 x 660 μL,  store at –15 to –25°C, protect from light
• E1A DNA Long Fragment Assay, 1 x 400 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 3 x 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

PrepSEQ Nucleic Acid Extraction Kit

• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
• Elution Buffer (1 bottle, 25 mL)
• Proteinase K Buffer( 1 bottle, 50 mL)
• Magnetic Particles (2 tubes, 1.5 mL/tube)
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL). Store at or below -20°C
• PK Buffer II (1 bottle, 11 mL)
• Store all at room temperature unless otherwise indicated

PrepSEQ Residual DNA Sample Preparation Kit

• Proteinase K (20 mg/mL) (1 tube, 1.25 mL)
• Yeast tRNA (10 mg/mL) (1 tube, 0.5 mL)
• Glycogen (5 mg/mL) (2 tubes, 1.0 mL/tube)
• Store all at or below -20°C