Applied Biosystems™ FlashTag™ Biotin HSR RNA Labeling Kits, 10 Reactions

Applied Biosystems™ FlashTag™ Biotin HSR RNA Labeling Kits, 10 Reactions

FlashTag™ Biotin HSR RNA Labeling Kits are high-sensitivity labeling reagents designed to prepare target from RNA samples for GeneChip™ miRNA Arrays.

Features

• Easy-to-use: 45-minute assay—from RNA sample to labeled target—without amplification or purification steps
• Low sample input: Requires as little as 130 ng total RNA
• Variety of samples: Works with any intact, degraded, or FFPE RNA sample
• Built-in controls: Contains RNA spike control oligos for GeneChip miRNA Arrays

Superior performance:

• Reproducibility: Inter-and intra-lot signal correlation is typically R >0.95
• Sensitivity: Detects 94% of miRNA transcripts at 1.0 attomole
• Dynamic range: >3 logs
• Specificity: 1 nucleotide discrimination
  
  

Contents

• 10X Reaction Buffer
• 25mM MnCl2  
• ATP Mix
• PAP Enzyme
• 5X FlashTag Biotin HSR Ligation Mix .
• T4 DNA Ligase
• HSR Stop Solution –20°C
• RNA Spike Control Oligos
• ELOSA Spotting Oligos
• ELOSA Positive Control  
• Nuclease-Free Water
• 27.5% Formamide